Bio-Techne and Refeyn Launch Four-Hour, Nanogram-Level Bispecific Characterization Workflow
TECH•Bio-Techne Corporation and Refeyn launched an integrated four-hour workflow combining MauriceFlex icIEF fractionation with TwoMP mass photometry for single-molecule resolution of charge heterogeneity, molecular weight, and aggregation in bispecific antibodies and biosimilars. The process reduces sample requirements to nanogram levels, accelerating biosimilar development and cutting manufacturing risk.
1. Integrated Workflow Introduced
Bio-Techne and Refeyn unveiled a four-hour workflow that integrates MauriceFlex imaged capillary isoelectric focusing with TwoMP mass photometry to characterize charge variants, size distribution, and aggregation of bispecific antibodies and biosimilars in a single process.
2. Technical Capabilities
The combined system achieves single-molecule resolution using nanogram-level samples and directly correlates icIEF-resolved charge heterogeneity with molecular weight and aggregation, eliminating the need for multiple traditional assays.
3. Impact on Biosimilar Manufacturing
By reducing sample requirements and streamlining analysis, the workflow lowers development risk, accelerates process development, and supports regulatory submissions with high-resolution charge variant data.
4. Demonstrations and Presentations
The partners demonstrated the workflow on Mosunetuzumab-axgb and a biosimilar in a joint webinar and will showcase the approach at the 2026 ASMS conference, highlighting its potential to advance next-generation antibody development.
